大會邀請報告 Plenary Lectures

Life at the Single Molecule Level: from Single Molecule Enzymology to Single Cell Genomics Xiaoliang Sunney Xie Harvard University, Department of Chemistry and Chemical Biology Peking University, Biodynamic Optical Imaging Center DNA exists as single molecules carrying genetic codes in individual cells. For this reason gene expression is stochastic. Single-molecule gene expression experiments in live single cell, when combined with single molecule enzymology in vitro, allowed quantitative and mechanistic interpretations of the fundamental processes of gene expression. The fact that there are 46 different individual DNA molecules (chromosomes) in a human cell dictates that genomic variation occurs stochastically and cannot be synchronized among cells. Probing such genomic variations necessitates single-cell single-molecule measurements, which have been made possible recently, opening exciting opportunities to investigate and diagnose cancer and to avoid genetic disorders in newborns.